Figure 10.

Identification of proteins in urine from Fanconi patients. Twenty microliters of urine samples from two healthy subjects (lanes 1, 2, 5, 6) and 5 μl of urine samples from two patients with multiple myeloma (lanes 3, 4, 7, 8 ) were subjected to 4 to 15% nonreducing SDS-PAGE and transfer to nitrocellulose membranes. Replicate filters were either stained with Ponceau S (lanes 1–4) or analyzed by immunoblot analysis using polyclonal antisera against the indicated proteins (lanes 5–8). The asterisks denote immunoglobulin light chain monomers and dimers present in urine from patients with multiple myeloma. Similar results were seen in three additional patients with multiple myeloma (not shown).