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SV40 large T antigen expression in MITC. Northern blot revealed a 2.5-kb mRNA band corresponding to the SV40 large T antigen in MITC and Cos7 cells (positive control). An 18 S probe was used to check the quality of the RNA. Indirect immunofluorescence using anti-SV40 large T antibody was clearly positive in MITC cells. These experiments were repeated three times at different subcultures.
