Figure 7.
Existence of an ACh-induced current in MITC cells. A: Representative whole-cell recording of ACh-induced inward current at a holding potential of −80 mV. Arrow indicates zero current. This experiment was repeated on 12 cells. B: Typical effect of ACh on the steady-state current-voltage relationship. Current traces obtained in control conditions, during application of external medium containing ACh to the cell, and after washout. The intersection of the control trace with the x axis gives a resting membrane potential of about −50 mV, whereas the intersection of the control trace with the ACh trace gives a reversal potential (EACh) of the ACh-induced current of about −2 to −4 mV. This experiment was performed on six cells from different subcultures.