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Only nAChRs are functional in MITC cells. A: Representative whole-cell ACh-induced inward current in the presence of atropine. B: Complete blockade of ACh-induced current by α-bungarotoxin (α-BgT) and total absence of muscarin-induced current. C: Partial recovery of a completely blocked nicotine-activated current by α-BgT during successive short applications of nicotine. Holding potential: −80 mV. Three different cells were used.
