Table 1.
Characteristics of the Oligonucleotides Used in This Study
| Oligonucleotide | Name | Strand | Sequence 5′ 3′ | Hybridization temperature (°C) | Expected size (bp) |
|---|---|---|---|---|---|
| α-chain | A1 | − | GGCTCCGAACATGAGACCCG | 57 | 704 and 629* |
| A2 | + | GAAGCAGTACGTCGCGGACG | 57 | ||
| β-chain | B1 | − | GTGTCAGGGTCAGCGTTGGT | 60 | 579 |
| B2 | + | TGCGGCGGATGATGAGGTAG | 60 | ||
| δ-chain | D1 | − | GCCCTCACACTCTCCAACCT | 62 | 468 |
| D2 | + | TCTCCCACTCCCCGTTCTCT | 62 | ||
| γ-chain | G1 | − | GAAGCCCTCACCACCAATGT | 62 | 513 |
| G2 | + | GTAGGTGAAGGAAGGACGGT | 62 | ||
| ε-chain | E1 | − | CGAGGAACTGCGTCTTTATC | 56 | 644 |
| E2 | + | CGGATGATGAGCGAGTAGAT | 56 | ||
| GAPDH | − | ATCACCATCTTCCAGGAGCG | 62 | 574 | |
| + | CCTGCTTCACCACCTTCTTG | 62 |
*Expected size for P3A+ and P3A− α-subunit isoforms, respectively.