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CCND1 mRNA is alternately spliced in SCCHN patients with different CCND1 A/G870 genotypes. The expression of CCND1 transcript a (A) and CCND1 transcript b (B) was detected by PCR on cDNA synthesized from tumors with CCND1 AA870 (lanes 1 and 2) CCND1 AG870 (lanes 3 and 4) and CCND1 GG870 (lanes 5 and 6) genotypes. PCR products were run on polyacrylamide gels and silver stained. φX174 HinfI digest was used as a size marker.
