Figure 3.

CCND1 alleles modulate transcript splicing in head and neck tissues. Restriction fragment length polymorphism-PCR analysis was performed on cDNA synthesized from tumor tissues from three patients with the heterozygote CCND1 AG⁸⁷⁰ genotype (patient 1, lanes 1 and 2; patient 2, lanes 3 and 4; and patient 3, lanes 5 and 6) (A), and normal tissues from two of these patients (patient 1, lanes 1 and 2; patient 2, lanes 3 and 4) (B). CCND1 alleles were resolved through digestion of PCR products with ScrFI and separated on polyacrylamide gels and silver stained. CCND1 alleles are marked with arrows. ScrFI digests of CCND1 transcript a-specific PCR product show transcript a is spliced equally from CCND1 allele A⁸⁷⁰ and G⁸⁷⁰ alleles in both tumor (A: lanes 1, 3, and 5) and normal (B: lanes 2 and 4) tissues; whereas digests of CCND1 transcript b-specific PCR products show that transcript b is spliced mainly from CCND1 A⁸⁷⁰ allele in both tumor (A: lanes 2, 4, and 6) and normal (B: lanes 1 and 3) tissues.