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. 2001 Nov;159(5):1671–1679. doi: 10.1016/s0002-9440(10)63014-3

Figure 2.

Figure 2.

Separation of RT-PCR products for KIR and NKG2A by DNA analyzer. RT-PCR products for KIR and NKG2A were mixed with RT-PCR β-actin product in equal portions and separated by electrophoresis on the DNA analyzer. Four pairs of GeneScan tracings for a case of sinonasal lymphoma are shown, with the size of the PCR product on the x axis and the size of the peak on the y axis. These tracings are KIR 2D4L (A) and its negative control (B), KIR 2D (C) and its negative control (D), KIR 3D (E) and its negative control (F), and NKG2A (G) and its negative control (H). The expected sizes of the PCR products for KIR 2D4L, KIR 2D, KIR 3D, β-actin, and NKG2A are 83, 85, 90, 96, and 99, respectively. This case is KIR 2D4L+ (arrow in A), KIR 2D−, and KIR 3D−, and NKG2A+ (arrow in G). Note also a small peak of size 85, corresponding to KIR 2D, can be seen in D, and small peaks of size 96, corresponding to β-actin, can be seen throughout all of the negative controls (B, D, F, and H).