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. 2001 Nov;159(5):1815–1826. doi: 10.1016/S0002-9440(10)63028-3

Figure 5.

Figure 5.

Effects of CpG island DNA hypermethylation on GSTP1 promoter function in LNCaP prostate cancer (PCA) cells. A: Unmethylated GSTP1 promoter/CAT reporter constructs were used for GSTP1 promoter mapping, 33 revealing transcriptional enhancing sequences at −408 to −291 and at −73 to −65 5′ of the transcription start site after transfection into LNCaP PCA cells. When methylated GSTP1 promoter sequences (black dots), prepared by treatment with SssI methylase, were ligated to CAT reporter sequences and transfected into LNCaP PCA cells, a reduction in CAT reporter activity, in comparison to unmethylated GSTP1 promoter/CAT reporter-transfected LNCaP cells, was evident. B: The trans-activation effects of 5-aza-C exposure (black bars) on the activity of unmethylated CMV, SV2, and GSTP1 promoters in LNCaP PCA cells were assessed. 5-Aza-C treatment of unmethylated GSTP1 promoter/CAT reporter-transfected LNCaP cells triggered only minimal increases in GSTP1 promoter activity.