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Incorporation of radiolabeled amino acids into TGF-β1. Growth-arrested cells were stimulated under serum-free conditions with insulin (5 μg/ml) in the presence of 4 μCi of 3H-radiolabeled amino acid mixture. Supernatant samples were subsequently collected after 48 hours and TGF-β1 immunoprecipitated as described in Materials and Methods. After immunoprecipitation, radiolabeled TGF-β1 was visualized by autoradiography.
