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PDGF B-chain transcript levels in granulation tissue. RNA was extracted from thick sections of sponge granulation tissue at 6 and 8 weeks after implantation. PDGF B-chain transcript levels were determined by TaqMan real-time reverse transcriptase-polymerase chain reaction and normalized to level of 18s RNA. Results are the mean ± SEM of triplicate determinations of three to four sponges from each mouse, three mice/genotype.
