Figure 2.

Effect of sequence variation within southern populations on reporter gene activity in vivo. All constructs tested contain ≈1,000 bp Ldh-B 5′ regulatory sequence. Alleles are drawn from populations as follows: ME1, Maine; GA6, Georgia; FL1, Florida; NJ3, New Jersey. Activity is light units generated by Ldh-B firefly luciferase constructs relative to light units generated by prL-CMV (sea pansy luciferase) constructs (mean ± SE; n = 5 for each group). All southern (GA6, FL1, NJ3) constructs (open bars) promoted significantly lower expression than the northern (ME1) construct (shaded bar) and did not differ significantly from each other (P < 0.05 ANOVA followed by GT-2 multiple comparison of means).