Figure 3.

In vivo reporter gene activity promoted by 500- and 400-bp fragments of Ldh-B 5′ regulatory sequence. Open bars are constructs generated from a representative southern allele (FL1) containing either 400 or 500 bp of 5′ regulatory sequence. Filled bars are constructs generated from a representative northern allele (ME1) containing either 400 or 500 bp of 5′ regulatory sequence. (A) Injected into southern fish (from Florida). (B) Injected into northern fish (from New Hampshire). Activity is light units generated by Ldh-B firefly luciferase constructs relative to light units generated by prL-CMV (sea pansy luciferase) constructs (mean ± SE; n = 5). The 500-bp southern construct (indicated by *) is significantly different from all other constructs injected into southern or northern fish, respectively (P < 0.05, ANOVA followed by GT-2).