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Bisulfite-modified sequencing of the first intron of the S100A4 gene showing the methylation status of two CpG sites (+315, +331). NP 133 is a normal pancreas sample with 90% methylation; Hs766T pancreatic cancer cell line is completely methylated; and PC 19 is a primary pancreatic adenocarcinoma, labeled for S100A4 on immunohistochemistry, showing a 90% conversion of the cytosine residues to thymine.
