HPV16 E7 antigen in cervical GCs. Immunohistochemistry on paraffin-embedded sections of high-grade SIL with GCs was performed with antibody against HPV16 E7 antigen (a and b), antibody pre-incubated with GST-E7 (c and d), and pre-incubated with GST (e and f). g: another GC on the same slide as panel a, but negative for E7 immunostaining. h: E7 immunostaining of high-grade SIL but not of cervical stroma. The bar in b and c represent 50 μm. E, epithelium; S, stroma; GC, germinal center.