Figure 5.

CD36 mediates ROS production by microglia and H₂O₂ production by macrophages plated on fAβ-coated surfaces. A: N9 microglia (106/ml) were preincubated for 30 minutes with anti-CD36 monoclonal antibody SMφ or NL07 (20 μg/ml) or isotype-matched control antibody MOPC-104E (20 μg/ml). Cells (50,000) were added to each spot of multispot slides coated with fibrillar Aβ 1-42 (black bars). ROS production was measured using the NBT assay as described in Materials and Methods. B: Human monocyte-derived macrophages (4 to 6 days in culture) were preincubated with anti-CD36 mAbs (SMφ or NL07, 20 μg/ml) or TSP-1 (5 μg/ml), plated on surfaces coated with 2.5 μg of collagen IV and 2 μg of fibrillar Aβ 1-42 (black bars) or fibrillar Aβ 25-35 (gray bars) and assayed for H₂O₂ secretion as described in Materials and Methods. Data are mean values ± SEM of three experiments done in triplicate. ROS production in response to Aβ 42-1 was similar to background (see Figure 4B ▶ , and data not shown).