Figure 2.

Western immunoblot analysis of AR protein in CWR22 tumors. An AR protein of 110 to 114 kd was present in lysates of androgen-dependent CWR22 tumors from intact mice. AR protein decreased after castration until tumor recurred ∼150 days after castration. AR protein levels found in recurrent tumors in the absence of androgens were similar to those found in the original androgen-dependent tumors. The position of the molecular mass marker (kd) is indicated. This experiment was performed with two to six different tumors at each time point with similar results.