Table 4.
Induction of Valvulitis in Lewis Rats by Immunization with Cardiac Myosin, Myosin Subfragments, or LMM Peptides
| Immunogen | Valvulitis* (positive/total) |
|---|---|
| Whole molecule | |
| Human cardiac myosin | 10 /23 |
| Rat cardiac myosin | 6 /13 |
| Subfragments of HCM | |
| HMM | 4 /5 |
| S1 | 0 /4 |
| S2 | 2 /3 |
| Related molecules | |
| Human skeletal myosin | 0 /6 |
| Light meromyosin peptides | |
| LMM peptides 1–6 | 0 /6 |
| LMM peptides 7–12 | 0 /6 |
| LMM peptides 13–18 | 0 /6 |
| LMM peptides 19–24 | 0 /11 |
| LMM peptides 25–30 | 0 /6 |
| LMM peptides 31–36 | 2 /6 |
| LMM peptides 37–42 | 2 /4 |
| LMM peptides 43–49 | 2 /6 |
| Controls | |
| Bovine serum albumin | 0 /6 |
| PBS+ adjuvants | 0 /34 |
*Presence of inflammatory cells in valve tissue were indicated as shown in Figure 3 ▶ . The level of valvulitis was similar in peptide groups 31 to 36, 37 to 42, and 43 to 49. These peptide groups produced a similar valvulitis that was seen as cellular infiltration at the valvular surface endothelium. In some instances, the valvulitis was more severe for the whole cardiac myosin molecule.