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. 2002 Jan;160(1):247–254. doi: 10.1016/S0002-9440(10)64368-4

Figure 1.

Figure 1.

RT-PCR detection of RET/PTC rearrangements in TCV. TCV samples were analyzed for RET/PTC activation by RT-PCR. The reaction products were hybridized with a RET TK probe. The housekeeping HPRT mRNA was amplified for normalization. The results obtained with representative samples are shown. RNA extracted from PTC samples previously shown to carry a RET/PTC1 (lane PTC1+) or a RET/PTC3 (lane PTC3+) rearrangement served as positive controls. RNA from a thyroid neoplastic sample previously shown to be negative for RET/PTC1 and RET/PTC3 was used as a negative control (lane (-)). There was no amplification when samples did not undergo previous reverse transcription (lane –RT contains sample 12 amplified without previous reverse transcription). A schematic representation of the RET/PTC1 and RET/PTC3 rearrangements and of the primers used is shown.