Elastin staining of the sections of mouse heart isografts and allografts. Scale bars, 50 μm. A: A representative section from a B/6 isograft in the no-storage group, 8 weeks after transplantation. Intimal lesions rarely developed in the isografts in the no-storage group. B: A representative section from a B/6 isograft following 4-hour cold ischemia in saline, 8 weeks after transplantation. Note the mild intimal thickening. C: A representative section from a B/6 isograft in the no-storage group, 12 weeks after transplantation. D: A representative section from a B/6 isograft following 4-hour cold ischemia in Stanford solution, 12 weeks after transplantation. The incidence of GAD tended to increase following 4-hour cold ischemia in either the saline and Stanford solution groups; GAD lesions from the 4-hour cold ischemia group showed moderate to severe intimal thickening at 12 weeks. E: A representative section from a bm1 to B/6 allograft in the no-storage group, 12 weeks after transplantation. F: A representative section from a bm1 to B/6 allograft in the 4-hour cold ischemia group, 12 weeks after transplantation. Similar severe GAD lesions developed in MHC class I disparate allografts without cold ischemia. G: A representative section from a bm12 to B/6 allograft in the no-storage group, 8 weeks after transplantation. H: A representative section from a bm12 to B/6 allograft in 4-hour cold ischemia group, 8 weeks after transplantation. Similar severe GAD lesions developed in MHC class II disparate allografts without cold ischemia.