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. 2002 Mar;160(3):1077–1087. doi: 10.1016/S0002-9440(10)64928-0

Figure 2.

Figure 2.

Representative panels of RPA from transplanted hearts. Fifteen micrograms of total RNA from normal non-transplanted hearts, or from hearts explanted at 0 hour, 4 hour, 24 hour, 3 days, or 7 days after transplantation were analyzed using the multiprobe RPA kits mCK-3b (containing DNA templates for TNF-β, LT-β, TNF-α, IL-6, IFN-γ, IFN-β, TGF-β1, TGF-β2, TGF-β3, MIF, L32, and GAPDH) and mCK-2b (containing DNA templates for IL-12p35, IL-12p40, IL-10, IL-1α, IL-1β, IL-1Ra, IL-18, IL-6, IFN-γ, MIF, L32, and GAPDH). The lanes on the far left show the undigested labeled probes that serve as size references, and the lanes on the far right show the results of RPA using a control RNA sample that was supplied by a manufacturer of the kit. Left panel: Representative gel images of B/6 isografts experiment analyzed by mCK-3b. Right panel: Representative gel images of BALB/c to B/6 allografts experiment analyzed by mCK-3b.