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. 2002 Mar;160(3):1077–1087. doi: 10.1016/S0002-9440(10)64928-0

Figure 4.

Figure 4.

Immunohistochemical staining of representative sections from B/6 isografts. Scale bar, 50 μm. A: ICAM-1 staining of a section from a B/6 isograft in no storage group, at 4 hours after transplantation. ICAM-1 is weakly expressed in the endothelium of coronary vessels. B: Negative control staining for A using isotype-matched hamster IgG instead of the anti-ICAM-1 antibody. C: ICAM-1 staining of a section from a B/6 isograft in the cold ischemia group, 4 hours after transplantation. ICAM-1 was strongly expressed in isografts following 4-hour cold ischemia. D: Negative control staining for C. E: VCAM-1 staining of a section from a B/6 isograft in no storage group, 24 hours after transplantation. Endothelial VCAM-1 is increased by the transplant procedure, compared with normal heart. F: Negative control staining for E using isotype-matched rat IgG. G: VCAM-1 staining of a section from a B/6 isograft in 4-hour cold ischemia group, 24 hours after transplantation. Positive staining for VCAM-1 expression was seen in the majority of the vessels in isografts following 4-hour cold ischemia. H: Negative control staining for G. I: P-selectin staining of a section from a B/6 isograft in no storage group, 4 hours after transplantation. J: Negative control staining for I using isotype-matched rat. K: P-selectin staining of a section from a B/6 isograft in 4-hour cold ischemia group, 4 hours after transplantation. L: Negative control staining for K. M: E-selectin staining of a section from a B/6 isograft in no storage group, 4 hours after transplantation. N: Negative control staining for M. O: E-selectin staining of a section from a B/6 isograft in 4-hour cold ischemia group, 4 hours after transplantation. P: Negative control staining for O. P-selectin and E-selectin are sparsely expressed in isografts at any time point.