Table 3.
Differentially Expressed Genes in MT-Null and WT Mice Treated with Lead*
| Gene | MT-null control | MT-null lead 4000 ppm | MT-null lead/ control | WT control | WT lead 4000 ppm | WT lead/ control | |
|---|---|---|---|---|---|---|---|
| Stress-response proteins (first group) | Mean ± SEM | Mean ± SEM | Mean ± SEM | Mean ± SEM | |||
| Heat shock protein HSP27 | 1915 ± 2 | 4181 ± 891 | 2.18 | 3411 ± 364 | 3302 ± 934 | 0.97 | |
| Heat shock protein HSP84 | 10222 ± 4143 | 14301 ± 640 | 1.40 | 14991 ± 811 | 12755 ± 925 | 0.85 | |
| Microsomal GST (MGST1) | 4784 ± 596 | 8721 ± 306 | 1.82 | 6681 ± 27 | 7029 ± 1134 | 1.05 | |
| Glutathione S-transferase-5 (GST mu) | 14369 ± 1999 | 35330 ± 2752 | 2.46 | 33379 ± 6472 | 35428 ± 5084 | 1.06 | |
| Glutathione S-transferase theta 1 | 1409 ± 33 | 4213 ± 898 | 2.99 | 3216 ± 78 | 2909 ± 601 | 0.91 | |
| Glutathione S-transferase Pi | 7920 ± 1609 | 20892 ± 4302 | 2.64 | 17357 ± 7238 | 17715 ± 4877 | 1.02 | |
| Oxidative stress protein A170 | 2477 ± 67 | 4526 ± 419 | 1.83 | 2534 ± 211 | 2571 ± 154 | 1.26 | |
| Cell signaling and transducers (second group) | |||||||
| Insulin-like growth factor IGFB6 | 4675 ± 3115 | 10823 ± 2335 | 2.32 | 10567 ± 5353 | 11596 ± 3393 | 1.10 | |
| Insulin-like growth factor II IGF-II | 1792 ± 215 | 4275 ± 2500 | 2.39 | 3519 ± 3216 | 4701 ± 3062 | 1.34 | |
| Wingless MMTV integration WNT 3 | 3218 ± 1088 | 6683 ± 1962 | 2.07 | N.D. | N.D. | ||
| WNT5B | 2424 ± 260 | 4972 ± 655 | 2.05 | N.D. | N.D. | ||
| Tumor necrosis factor-beta | 744 ± 214 | 2744 ± 1913 | 3.68 | 524 ± 139 | 298 ± 156 | 0.57 | |
| Cytokine inducible CISH7 | 6492 ± 2264 | 10727 ± 2423 | 1.65 | 12660 ± 1620 | 13995 ± 3714 | 1.11 | |
| Nuclear factor-kappa B (IkB-beta) | 1626 ± 88 | 8941 ± 5194 | 5.45 | 1162 ± 9 | 1272 ± 418 | 1.09 | |
| DNA synthesis, repair (third group) | |||||||
| DNA excision repair protein ERCC1 | 711 ± 266 | 1840 ± 779 | 2.59 | N.D. | N.D. | ||
| DNA repair protein XPBC | 3170 ± 740 | 5819 ± 1410 | 1.84 | 2767 ± 201 | 5141 ± 1569 | 1.86 | |
| UV excision repair protein RAD23 | 4186 ± 1417 | 7080 ± 878 | 1.69 | 508 ± 389 | 1716 ± 1028 | 3.42 | |
| DNA polymerase delta POLD1 | 1324 ± 373 | 3903 ± 2341 | 2.95 | 1826 ± 763 | 1454 ± 173 | 0.80 | |
| DNAse 1 | 33264 ± 9000 | 21764 ± 4110 | 0.65 | 29969 ± 2862 | 28308 ± 3807 | 0.95 | |
| Apoptosis-associated proteins (fourth group) | |||||||
| Tumor necrosis factor receptor 1 | 1121 ± 155 | 4601 ± 1409 | 4.11 | 2043 ± 2007 | 1619 ± 1450 | 0.79 | |
| Tumor necrosis factor receptor 2 | 1018 ± 81 | 13492 ± 5031 | 13.3 | 3062 ± 1100 | 2382 ± 1060 | 0.78 | |
| Caspase-7 | 2858 ± 2734 | 245 ± 138 | 0.09 | 200 ± 61 | 75 ± 40 | 0.37 | |
| MCL-1 | 5178 ± 1850 | 2221 ± 540 | 0.43 | 2296 ± 270 | 3236 ± 509 | 1.40 | |
| Sentrin; ubiquitin-like protein | 7783 ± 1849 | 3532 ± 606 | 0.45 | 1484 ± 817 | 1654 ± 1454 | 1.11 | |
| Inhibitor of neuronal NO synthetase | 12867 ± 2830 | 8963 ± 1697 | 0.71 | 12010 ± 753 | 10204 ± 408 | 0.85 | |
| Cell surface antigens (fifth group) | |||||||
| Connexin 26 | 10096 ± 2018 | 4518 ± 1057 | 0.46 | 5944 ± 1841 | 7544 ± 2178 | 1.27 | |
| Connexin 40 | 2835 ± 210 | 4737 ± 712 | 1.67 | 2942 ± 531 | 7067 ± 2054 | 2.40 | |
| m-numb (m-NB) | 3592 ± 453 | 1642 ± 193 | 0.46 | 1688 ± 285 | 1464 ± 768 | 0.87 | |
| Glutamate receptor subunit | 2864 ± 1204 | 696 ± 493 | 0.24 | 1189 ± 519 | 1222 ± 513 | 1.03 | |
| Transcription factors (sixth group) | |||||||
| Msx-interacting Zn finger protein | 5069 ± 1976 | 1675 ± 842 | 0.33 | 2537 ± 944 | 3565 ± 1390 | 1.40 | |
| Brain-specific homebox (BRN-1) | 7308 ± 1780 | 3992 ± 727 | 0.55 | 4347 ± 931 | 3855 ± 1216 | 0.91 | |
| TBX2 protein | 4752 ± 1154 | 1980 ± 483 | 0.43 | 10279 ± 2927 | 8546 ± 4260 | 0.83 | |
| Homoboxcux2 (CUTL2) | 2918 ± 235 | 8661 ± 3018 | 2.97 | 3233 ± 1825 | 2496 ± 404 | 0.78 | |
| Eyes absent homolog2 | 4352 ± 2058 | 10837 ± 4030 | 2.49 | 12694 ± 1847 | 11597 ± 5639 | 0.91 | |
| Transcriptional coactivator of AML-1 | 5533 ± 1372 | 2119 ± 1126 | 0.38 | 11081 ± 1791 | 7959 ± 3366 | 0.72 | |
| Transcription factor 3B | 6794 ± 202 | 3160 ± 596 | 0.47 | 6085 ± 1097 | 4219 ± 1330 | 0.69 | |
| Ret finger protein | 599 ± 15 | 1842 ± 638 | 3.10 | 906 ± 371 | 1565 ± 899 | 1.73 | |
| Trans-acting transcription factor 3 | 4190 ± 670 | 1545 ± 794 | 0.37 | 1724 ± 805 | 569 ± 184 | 0.33 | |
| A T motif-binding factor (ATBF1) | 14324 ± 2979 | 25596 ± 6946 | 1.79 | 23293 ± 3372 | 21088 ± 76826 | 0.91 | |
| D-binding protein (DBP) | 10812 ± 2609 | 169 ± 316 | 0.29 | 2604 ± 41 | 3512 ± 1418 | 1.34 | |
| Cell cycle Regulators (seventh group) | |||||||
| Protein kinase-β cAMP dependent | 6192 ± 1787 | 3016 ± 464 | 0.49 | 2421 ± 1045 | 2558 ± 1303 | 1.06 | |
| G2/M-specific cyclin G | 12799 ± 3658 | 8715 ± 3360 | 0.68 | 9080 ± 1522 | 10282 ± 4090 | 1.13 | |
| Extracellular signal regulator ERK1 | 5473 ± 380 | 2126 ± 663 | 0.39 | 1981 ± 765 | 2312 ± 1011 | 1.16 | |
| T ob antiproliferative factor | 11180 ± 938 | 7322 ± 1260 | 0.66 | 2039 ± 77 | 2793 ± 969 | 1.36 | |
| Cell adhesion receptors and proteins (eighth group) | |||||||
| α-E-catenin | 9458 ± 2098 | 5941 ± 1800 | 0.63 | 8413 ± 1211 | 8876 ± 852 | 1.05 | |
| Cell surface glycoprotein MAC-1 | 1840 ± 228 | 5140 ± 1516 | 2.79 | 3498 ± 444 | 6519 ± 2306 | 1.86 | |
| Integrin alpha 6 | 3516 ± 844 | 2268 ± 601 | 0.65 | 3188 ± 390 | 2943 ± 570 | 0.92 | |
| Oncogenes and tumor suppressors (nineth group) | |||||||
| Transcription termination factor TTF1 | 11446 ± 1103 | 5614 ± 2085 | 0.49 | 521 ± 515 | 779 ± 308 | 1.49 | |
| Tumor susceptibility protein TSG101 | 3440 ± 840 | 1844 ± 380 | 0.54 | 3836 ± 836 | 2879 ± 1610 | 0.75 | |
| c-myc oncogene | 464 ± 105 | 1145 ± 110 | 2.47 | 537 ± 299 | 774 ± 176 | 1.44 | |
| Erin; Villin 2 | 9229 ± 1202 | 5792 ± 1039 | 0.63 | 7936 ± 1436 | 6553 ± 1582 | 0.83 | |
| c-met oncogene | 10622 ± 3924 | 6705 ± 1681 | 0.63 | 8104 ± 945 | 8506 ± 1464 | 1.05 | |
| Retinoblastoma-like protein RBL2 | 1263 ± 149 | 2851 ± 1053 | 2.26 | 1330 ± 525 | 2125 ± 855 | 1.60 | |
| Ion channels and transporters (tenth group) | |||||||
| Glutamate receptor, ionotropic NMD2A | 618 ± 77 | 4697 ± 1691 | 6.31 | 2533 ± 1059 | 1636 ± 721 | 0.65 | |
| P-glycoprotein (MDR1) | 1237 ± 50 | 3277 ± 952 | 2.65 | 3052 ± 674 | 2055 ± 844 | 0.67 | |
| Receptors (eleventh group) | |||||||
| IL-6 receptor alpha | 553 ± 141 | 4217 ± 1750 | 7.62 | 1891 ± 393 | 1841 ± 120 | 0.99 | |
| 5-hydroxytryptamine receptor | 1066 ± 824 | 11149 ± 9102 | 10.5 | N.D. | N.D. | ||
| Serine protease inhibitor 1-2 | 4486 ± 610 | 17023 ± 1151 | 3.79 | 10910 ± 5711 | 13300 ± 5171 | 1.22 | |
*The data are mean ± SEM of four separate experiments from MT-null and WT mice treated with lead.
N.D., not detected.