Figure 1.

A: Immunoblotting for eNOS in skeletal muscle. Protein expression of eNOS in skeletal muscle was significantly higher in eNOS-Tg mice than WT mice. B: Immunoblots were analyzed and quantified by densitometry. Open and solid bars indicate the values of WT and eNOS-Tg mice, respectively. C: Ca²⁺-dependent NOS activity in skeletal muscle. Ca²⁺-dependent NOS activity in skeletal muscle was also significantly higher in eNOS-Tg mice than WT mice. Enzyme activity was expressed as citrulline production in picomole per milligram of protein per minute. D: NO release from ex vivo skeletal muscle. Acetylcholine-stimulated NO release from ex vivo skeletal muscle was assessed by measuring nitrite production with the use of a NOx analyzer. *P <0.05 vs. the value for WT mice.