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In situ superoxide detection in the reperfused vessels of skeletal muscle. In situ superoxide detection with dihydroethidium fluorescence in blood vessels of ischemia-reperfused (a–c) and non-ischemic control muscle (d–f) from WT (a, d), eNOS-Tg mice (b, e), and eNOS-Tg mice treated with L-NAME (c, f) at 60 minutes after reperfusion. Scale bar, 20 μm.
