Table 2.
Transcriptional Activation of TSG-6 Promoter in Response to TNF-α and PGE2
| Treatment | RLU × 10−3 (mean ± SE) |
|---|---|
| Control | 35.71 ± 2.1 |
| TNF-α (10 ng/ml) | 22.71 ± 2.9 |
| PGE2 (10 μM) | 64.42 ± 3.6 |
Effect of TNF-α and PGE2 on 1.32-kb TSG-6 promoter activity in hCSMCs. Transfection and cell stimulation were performed as described in Materials and Methods. The relative luciferase units (RLU) presented are from three independent experiments, each conducted with triplicate cultures. Values with different superscripts are significantly different (P < 0.05) by the Tukey-Kramer test.