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Enhanced adipogenesis of 3T3-F442A cells and de novo adipogenesis by Matrigel in combination with bFGF. 3T3-F442A preadipocytes were suspended in Matrigel with (D) or without (B) 1 μg/ml bFGF, and 100 μl of the cell suspension containing 2 × 106 cells was s.c. injected into a BALB/c nude mouse over the chest. As the control, 100 μl of Matrigel with (C) or without (A) 1 μg/ml bFGF also was injected. Arrows indicate fat pads formed 5 weeks after the injection.
