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Dependency of de novo adipogenesis on bFGF dose. Six-week-old mice were s.c. injected with Matrigel containing 0, 1, 10, 100, or 1,000 ng/ml bFGF (two injections into three mice) over the chest. (A) Averaged weight with SE of the Matrigel plugs excised 5 weeks after the injection. (B) Histological staining with hematoxylin and eosin of the tracts formed by injecting Matrigel containing 1 (a), 10 (b), 100 (c), or 1,000 (d) ng/ml bFGF. (Bar = 100 μm.)
