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. 2002 Apr;160(4):1481–1486. doi: 10.1016/S0002-9440(10)62573-4

Figure 2.

Figure 2.

MI and LOH analysis in Pten+/−/Mlh1−/− mouse. a: MI analysis on genomic DNA with U12235 locus. Lane 1: Undiluted tail DNA. Lanes 2 and 3: Two diluted DNA samples from the same mouse showing instability. b: MI analysis on uterine lesions with U12235. Lane 1: Microdissected DNA from normal myometrium. Lanes 2 and 3: DNA from two microdissected CAH lesions from the same mouse showing a shift in lane 3 but not definitively in lane 2. c: PCR-based analysis of LOH of Pten in endometrial lesions from Pten+/−/Mlh1−/− mice. Lane M: One-kb ladder. Lane 1: Amplification of tail DNA from Pten+/− mouse showing both mutant and wild-type alleles. Lane 2: Amplification of tail DNA from Pten+/+ mouse. Lane 3: Amplification of DNA from a microdissected lesion without LOH. Lanes 4 and 5: Amplification of DNA from two microdissected lesions showing LOH of the wild-type allele.