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PNA signals from LNCaP subline panel containing different telomere lengths. Four LNCaP sublines possessing different telomere lengths were fixed in formalin and embedded in a single paraffin block. Sections were stained for telomeres as described in Materials and Methods. Top: Cy3-PNA telomere probe fluorescence (oil objective; original magnification, ×1000). Bottom: DAPI fluorescence. Average telomere lengths (in kbp) calculated from TRF-Southern data are indicated.
