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. 2002 Apr;160(4):1445–1455. doi: 10.1016/S0002-9440(10)62570-9

Figure 6.

Figure 6.

Expression of CCR7 on liver-derived and peripheral blood lymphocytes. A: CCR7 expression on peripheral blood and liver-derived lymphocytes from an organ donor without liver disease. B: CCR7 expression on peripheral blood and liver-derived lymphocytes from a patient with PSC. A significant proportion of intrahepatic lymphocytes are CCR7+. C: The percentage of CD3+ T cells that express CCR7 is shown for peripheral blood and liver-derived lymphocytes from organ donors without liver disease (normal) and patients with PSC. D: The proportion of CD3+/CCR7+ T cells that express CD45RA for normal and PSC T cells is shown. E: To determine the nature of the CD45RA+ T cells in the liver fresh liver-derived lymphocytes were gated on CD3 and analyzed for expression of LFA-1. In blood (top) the majority of CD45RA+ T cells are LFA-1low confirming that they are naïve cells whereas in liver-derived cells the majority are LFA-1high and thus likely to be primed/memory cells (bottom). Representative staining is shown for matched blood and liver-derived lymphocytes from a patient with PSC, an organ donor (normal), and blood from a patient with UC. Cells are gated on lymphocytes by forward/side scatter and on CD3 staining. F: Immunostaining for CCR7+ lymphocytes within PSC liver tissue revealed occasional lymphocytes associated with areas of inflammation (white arrows) as well as positive cells with the morphology of DCs (black arrow). Staining was weak but two different anti-CCR7 monoclonal antibody 3D9 and 4H12 both detected positive cells.