Table 1.
Highly Expressed Genes Identified in Pancreatic Cancer Cell Lines and Tissues
| Affymetrix fragment name | Known gene name | Fold change | P value | eNorthern pattern* | SAGE normal tags† | Reported in pancreas | Ref. | Cellular location§ |
|---|---|---|---|---|---|---|---|---|
| 39829_at | ADP-ribosylation factor-like 7 | 7.17 | <0.00001 | A | 0,0 | no | C | |
| 37403_at | Annexin A1 | 5.66 | <0.00001 | A | 1,0 | no | C | |
| 89917_at | Apolipoprotein C-I | 8.09 | 0.00008 | B | 1,1 | yes | 11 | EM,M |
| 88518_at | Aspartate beta-hydroxylase | 5.59 | <0.00001 | A | 1,2 | no | C | |
| 1898_at | Ataxia-telangiectasia group D-associated protein | 5.21 | <0.00001 | A | 0,0 | no | C | |
| 91017_at | Baculoviral IAP repeat-containing 3 | 7.5 | <0.00001 | A | 0,0 | no | ||
| 74989_at | Biglycan | 12.27 | 0.00011 | B | 0,0 | yes | 36 | EM |
| 36976_at | Cadherin 11, type 2, OB-cadherin (osteoblast) | 5.93 | <0.00001 | B | 0,0 | no | M | |
| 38391_at | Capping protein (actin filament), gelsolin-like | 5.08 | <0.00001 | B | 4,1 | no | C | |
| 74707_at | Capping protein (actin filament), gelsolin-like | 11.66 | <0.00001 | B | 4,1 | no | C | |
| 53708_at | Cation-chloride cotransporter-interacting protein | 5.42 | <0.00001 | A | 2,0 | no | M | |
| 339_at | Caveolin 2 | 5.95 | <0.00001 | A | 0,0 | no | C | |
| 2036_s_at | CD44 antigen | 5.3 | <0.00001 | A | 0,2 | yes | 37 | M |
| 89856_at | CD83 antigen | 6.51 | <0.00001 | B | 0,0 | no | M | |
| 38112_g_at | Chondroitin sulfate proteoglycan 2 (versican) | 5.04 | 0.00009 | B | 0,0 | no | EM | |
| 46260_at | Claudin 1 | 5.61 | <0.00001 | A | 0,0 | no | M | |
| 35474_s_at | Collagen, type I, alpha 1 | 7.07 | 0.00002 | B | 1,1 | yes | 9;10 | EM |
| 32305_at | Collagen, type I, alpha 2 | 8.84 | 0.00001 | B | 4,2 | yes | 9;10 | EM |
| 32306_g_at | Collagen, type I, alpha 2 | 5.3 | 0.00128 | B | 4,2 | yes | 9;10 | EM |
| 63596_f_at | Collagen, type I, alpha 2 | 5.69 | 0.00286 | B | 4,2 | yes | 9;10 | EM |
| 60071_s_at | Collagen, type I, alpha 2 | 5.18 | 0.0048 | B | 4,2 | yes | 9;10 | EM |
| 37892_at | Collagen, type XI, alpha 1 | 6.88 | 0.00001 | B | 0,0 | yes | 9;10 | EM |
| 73132_r_at | Cyclin-dependent kinase inhibitor 2A (p16) | 5.87 | <0.00001 | A | 0,0 | yes | 38 | C |
| 40490_at | DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21 | 8.31 | <0.00001 | A | 8,9‡ | no | ||
| 73128_at | Dihydropyrimidinase-like 3 | 8.33 | 0.00001 | B | 1,0 | no | ||
| 39959_at | Diubiquitin | 5.49 | 0.00001 | B | 0,0 | no | C | |
| 48740_s_at | DKFZP564G013 protein | 6.61 | <0.00001 | A | 0,0 | no | ||
| 37981_at | Drebrin 1 | 5.24 | <0.00001 | A | 0,1 | no | C | |
| 1379_at | EphA2 | 5.07 | <0.00001 | A | 7,6‡ | no | M | |
| 56226_at | Eukaryotic translation initiation factor 2C, 2 | 5.84 | <0.00001 | A | 1,1 | no | C | |
| 34678_at | fer-1 (C. elegans)-like 3 (myoferlin) | 9.03 | <0.00001 | A | 0,0 | no | M | |
| 39945_at | Fibroblast activation protein, alpha | 5.57 | 0.00001 | B | 0,0 | no | M | |
| 311_s_at | Fibronectin 1 | 6.53 | 0.00005 | B | 2,1 | no | M,EM | |
| 65830_at | FXYD domain-containing ion transport regulator 5 | 8.69 | <0.00001 | A | 5,7‡ | no | ||
| 91306_s_at | Gap junction protein, beta 2, 26kD (connexin 26) | 7.32 | <0.00001 | A | 0,0 | yes | 39 | M |
| 91309_r_at | Gap junction protein, beta 2, 26kD (connexin 26) | 5.37 | <0.00001 | A | 0,0 | yes | 39 | M |
| 89908_f_at | GDP dissociation inhibitor 1 | 7.58 | <0.00001 | A | 0,1 | yes | 40 | C |
| 40365_at | Guanine nucleotide binding protein (G protein), alpha 15 | 5.18 | <0.00001 | A | 0,0 | no | ||
| 43366_at | Hypothetical protein FLJ10261 | 5.61 | <0.00001 | A | 0,1 | no | ||
| 43963_at | Hypothetical protein FLJ10261 | 6.35 | <0.00001 | A | 0,1 | no | ||
| 58235_at | Hypothetical protein FLJ10540 | 5.45 | <0.00001 | A | 0,0 | no | ||
| 44062_at | Hypothetical protein FLJ10849 | 5.59 | <0.00001 | A | 2,1 | no | ||
| 85285_at | Hypothetical protein FLJ11183 | 5.6 | <0.00001 | A | 0,0 | no | ||
| 54030_at | Hypothetical protein FLJ20373 | 7.08 | <0.00001 | A | 1,1 | no | ||
| 47427_at | Hypothetical protein FLJ20539 | 5.8 | <0.00001 | A | 0,0 | no | ||
| 74810_s_at | Hypothetical protein FLJ22569 | 13.05 | <0.00001 | A | 2,0 | no | ||
| 75276_at | Hypoxia-inducible factor 1, alpha subunit | 5.66 | <0.00001 | A | 1,0 | yes | 41 | C |
| 37558_at | IGF-II mRNA-binding protein 3 | 5.91 | <0.00001 | A | 1,1 | yes | 42 | |
| 88957_at | Integrin, beta-like 1 (with EGF-like repeat domains) | 7.49 | <0.00001 | A | 0,0 | no | M | |
| 89882_at | Interferon induced transmembrane protein 1 (9-27) | 6.51 | <0.00001 | A | 0,0 | yes | 43 | M |
| 35372_r_at | Interleukin-8 | 6.53 | <0.00001 | A | 0,0 | yes | 35 | C |
| 1369_s_at | Interleukin-8 | 5.14 | 0.00004 | A | 0,0 | yes | 35 | C |
| 41294_at | Keratin 7 | 10.77 | <0.00001 | A | 2,4 | yes | 18 | C |
| 33893_r_at | KIAA0470 gene product | 5.45 | <0.00001 | A | 0,0 | no | ||
| 35832_at | KIAA1077 protein | 6.99 | 0.00001 | B | 0,0 | no | ||
| 57215_at | KIAA1078 protein | 5.3 | <0.00001 | A | 1,1 | no | ||
| 36070_at | KIAA1199 protein | 5.61 | <0.00001 | A | 1,2 | no | ||
| 50402_at | KIAA1265 protein | 5.05 | <0.00001 | A | 0,0 | no | ||
| 60289_at | KIAA1323 protein | 5.18 | <0.00001 | A | 0,0 | no | ||
| 77022_at | KIAA1363 protein | 5 | <0.00001 | A | 0,0 | no | ||
| 41438_at | KIAA1451 protein | 5.83 | <0.00001 | A | 0,1 | no | ||
| 75014_i_at | KIAA1533 protein | 6.22 | 0.00002 | A | 93,123‡ | no | ||
| 78484_at | KIAA1577 protein | 5.36 | <0.00001 | A | 0,0 | no | ||
| 74535_at | Lamin B2 | 11.53 | <0.00001 | A | 0,0 | no | N | |
| 35280_at | Laminin, gamma 2 | 5.28 | <0.00001 | A | 0,0 | yes | 44 | EM |
| 91124_i_at | Leukemia-associated phosphoprotein p18 (stathmin) | 6.69 | <0.00001 | A | 5,3 | no | ||
| 32821_at | Lipocalin 2 (oncogene 24p3) | 8.86 | <0.00001 | A | 5,2 | yes | 45 | S |
| 73002_at | Matrix metalloproteinase 14 (membrane-inserted) | 7.27 | 0.00003 | B | 0,0 | yes | 11 | M |
| 668_s_at | Matrix metalloproteinase 7 (matrilysin, uterine) | 8.79 | 0.00001 | B | 0,0 | yes | 9 | S |
| 75026_s_at | Methylene tetrahydrofolate dehydrogenase | 5.97 | <0.00001 | A | 14,14‡ | no | C | |
| 35694_at | Mitogen-activated protein kinase kinase kinase kinase 4 | 5.6 | <0.00001 | A | 0,0 | no | C | |
| 38272_at | MKP-1 like protein tyrosine phosphatase | 5.63 | <0.00001 | B | 0,0 | no | ||
| 37032_at | Nicotinamide N-methyltransferase | 5.04 | 0.00056 | A | 0,2 | no | C | |
| 78518_at | Nuclear receptor subfamily 2, group F, member 1 | 7.48 | <0.00001 | A | 0,0 | no | C | |
| 75321_f_at | Nucleosome assembly protein 1-like 1 | 5.4 | <0.00001 | A | 0,3 | no | C | |
| 73229_at | Nucleosome assembly protein 1-like 1 | 6.29 | <0.00001 | A | 0,3 | no | C | |
| 91187_s_at | Nucleosome assembly protein 1-like 1 | 8.57 | <0.00001 | A | 0,3 | no | C | |
| 91189_r_at | Nucleosome assembly protein 1-like 1 | 8.48 | <0.00001 | A | 0,3 | no | C | |
| 91546_r_at | Nucleosome assembly protein 1-like 1 | 7.54 | <0.00001 | A | 0,3 | no | C | |
| 1451_s_at | Osteoblast specific factor 2 (fasciclin I-like) | 5.3 | 0.00025 | B | 0,0 | no | ||
| 78711_at | Paraneoplastic antigen MA1 | 5.49 | <0.00001 | A | 0,1 | no | N | |
| 81926_at | Peptidylarginine deiminase type I | 5.87 | <0.00001 | A | 0,0 | no | ||
| 37310_at | Plasminogen activator, urokinase | 5.71 | <0.00001 | A | 1,2 | yes | 46 | S |
| 189_s_at | Plasminogen activator, urokinase receptor | 6.18 | <0.00001 | A | 0,1 | yes | 46 | M |
| 74696_r_at | PDGF receptor, beta polypeptide | 6.14 | 0.00005 | B | 0,0 | yes | 47 | M |
| 91311_at | Pleckstrin homology-like domain, family A, member 1 | 14.66 | <0.00001 | A | 5,2 | no | ||
| 90442_at | Plectin 1, intermediate filament binding protein, 500kD | 6.69 | 0.00015 | A | 0,1 | no | C | |
| 49666_s_at | PRO1073 protein | 6.27 | <0.00001 | A | 1,5 | no | ||
| 63958_at | Prostate stem cell antigen | 5.34 | 0.00001 | B | 0,0 | yes | 6 | M |
| 40078_at | Protease, serine, 23 | 5.62 | <0.00001 | A | 0,0 | yes | 48 | |
| 40079_at | Protease, serine, 23 | 7.07 | <0.00001 | A | 0,0 | yes | 48 | |
| 80688_at | Protein kinase C-like 1 | 5.23 | <0.00001 | A | 0,1 | yes | 49 | C |
| 80463_at | Putative protein | 5.05 | <0.00001 | A | 0,2 | no | ||
| 46683_at | RAB6 interacting, kinesin-like (rabkinesin6) | 5.09 | <0.00001 | A | 0,0 | no | C | |
| 49125_at | Ras GTPase activating protein-like | 7.01 | <0.00001 | A | 4,4 | no | ||
| 49125_at | Ras GTPase activating protein-like | 7.01 | <0.00001 | A | 4,4 | no | ||
| 33730_at | Retinoic acid induced 3 | 5.53 | <0.00001 | A | 3,1 | no | M | |
| 57027_at | Retinoic acid induced 3 | 9.25 | <0.00001 | A | 3,1 | no | M | |
| 52123_at | Rho guanine nucleotide exchange factor (GEF) 1 | 5.35 | <0.00001 | A | 0,0 | no | C | |
| 89969_at | Ribosomal protein S15a | 5.55 | <0.00001 | A | 0,0 | no | C | |
| 74736_f_at | RNA binding motif, single stranded interacting protein 1 | 9.41 | <0.00001 | A | 0,0 | no | N | |
| 39421_at | Runt-related transcription factor 1 (aml1 oncogene) | 5.92 | <0.00001 | A | 0,4 | no | N | |
| 34319_at | S100 calcium-binding protein P | 8.73 | <0.00001 | A | 0,0 | no | N | |
| 74815_at | Secreted phosphoprotein 1 (osteopontin) | 7.98 | 0.01276 | B | 0,0 | no | S | |
| 38125_at | Serine (or cysteine) proteinase inhibitor, clade E, member 1 | 6.68 | <0.00001 | A | 10,10‡ | yes | 27 | |
| 39166_s_at | Heat shock protein 47 | 6.41 | <0.00001 | B | 1,4 | no | ||
| 41544_at | Serum-inducible kinase | 6.23 | <0.00001 | A | 0,0 | no | ||
| 39070_at | Singed (Drosophila)-like (sea urchin fascin homolog like) | 13.31 | <0.00001 | A | 1,1 | no | C | |
| 33143_s_at | Solute carrier family 16, member 3 | 8.23 | <0.00001 | A | 0,0 | no | M | |
| 87860_s_at | Solute carrier family 21, member 12 | 8.92 | <0.00001 | A | 0,0 | no | ||
| 32186_at | Solute carrier family 7, member 5 | 6.27 | <0.00001 | A | 0,0 | no | M | |
| 658_at | Thrombospondin 2 | 6.94 | 0.00001 | B | 0,0 | no | EM | |
| 659_g_at | Thrombospondin 2 | 9.92 | <0.00001 | B | 0,0 | no | EM | |
| 43353_at | Thrombospondin 2 | 5.37 | 0.00006 | B | 0,0 | no | EM | |
| 43353_at | Tissue inhibitor of metalloproteinase 1 | 8.14 | <0.00001 | A | 22,24‡ | yes | 28 | S |
| 74096_at | Topoisomerase (DNA) II alpha (170kD) | 5.28 | 0.00001 | A | 1,0/2,0 | no | N | |
| 69053_at | Transcription factor BMAL2 | 7.03 | <0.00001 | A | 0,0 | no | N | |
| 231_at | Transglutaminase 2 | 5.22 | <0.00001 | A | 5,11‡ | yes | 50 | C |
| 41531_at | Transmembrane 4 superfamily member 1 | 6.19 | <0.00001 | A | 7,5‡ | no | M | |
| 892_at | Transmembrane 4 superfamily member 1 | 6.22 | <0.00001 | A | 7,5‡ | no | M | |
| 46644_at | Transmembrane, prostate androgen induced RNA | 5.33 | <0.00001 | A | 1,2 | no | ||
| 91095_s_at | Transmembrane, prostate androgen induced RNA | 9.54 | <0.00001 | A | 1,2 | no | ||
| 31888_s_at | Tumor suppressing subtransferable candidate 3 | 5.99 | <0.00001 | A | 9,11‡ | no | C | |
| 291_s_at | Tumor-associated calcium signal transducer 2 | 9.29 | <0.00001 | A | 0,0 | yes | 10 | M |
| 82782_at | Zinc finger protein 267 | 5.53 | <0.00001 | A | 0,0 | no | C |
*A, elevated expression in cell lines and tumor tissues; B, elevated expression in tumor tissues only.
†Values listed are the total number of tags present in the two SAGE libraries of normal pancreatic duct epithelium (HX and H126) for each known gene identified. Genes with >5 tags present in at least one of the two libraries are indicated by an ‡, and were excluded from further analyses.
§Putative location. C, cytoplasmic; M, cell membrane; EM, extracellular matrix; N, nuclear; S, secreted.