Figure 3.

Immunolocalization of RelA in bronchiolar epithelial cells of mice after sensitization and challenge with allergen. Control mice were unmanipulated, sensitized mice received an intraperitoneal injection of OVA at days 1 and 14. Challenged mice received an intraperitoneal injection of vehicle at days 1 and 14 and were nebulized with aerosolized OVA at days 21, 22, and 23. Paraffin-embedded lung sections were prepared 48 hours after allergen challenge and stained with an antibody directed against RelA followed by incubation with an Oregon Green-conjugated secondary antibody. Propidium iodide was used as a nuclear counterstain to evaluate nuclear presence of RelA. Sections were scanned by confocal microscopy. Data are representative of results from two separate experiments.