Figure 5.

IKK activity assay from whole lungs of mice following sensitization and single challenge with allergen. Mice received an intraperitoneal injection of OVA at days 1 and 14 and were nebulized with aerosolized OVA at day 21. Control mice were sensitized but not exposed to aerosolized OVA. Mice were euthanized at various times following a single allergen challenge, the IKK complex was immunoprecipitated from lung homogenates and subjected to an in vitro kinase assay to measure the incorporation of ³²P into GST-IκBα. Presented data are representative of results from two separate experiments, from which band intensities were quantitated by phosphoimage analysis and are presented as fold-induction ± SEM.