Figure 7.

Luciferase activity in lungs from sensitized NF-κB/luciferase mice following challenge with aerosolized allergen. NF-κB/luciferase mice received an intraperitoneal injection of OVA at days 1 and 14 and were nebulized with aerosolized OVA at day 21 or at days 21, 22, and 23 (3× Ova). Control mice were sensitized but not exposed to aerosolized OVA. Lungs were harvested at 6 or 24 hours following a single challenge (Ova 6 hours and Ova 24 hours) or 48 hours following three aerosolized challenges (3× Ova 48 hours), and homogenates were assessed for luciferase reporter activity, which was normalized to total protein. Data are from three mice per group and are expressed as means ± SEM.