Figure 1.

Skp2 expression in lymphoid cell lines and normal tissues. A: Western blot analysis on human lymphoid cell lines. Cells from the indicated cell lines were lysed and Western blotting was performed as described in Material and Methods. Lane 1, Namalwa cell line; lane 2, DHL cell line; lane 3, recombinant His-tagged Skp2 protein. B–E: Formalin-fixed, paraffin-embedded tissue sections were stained by immunohistochemistry with an anti-Skp2 mAb, as described in Materials and Methods. In the thymus (B) and in the bone marrow (C), Skp2 stained primarily the immature subcortical thymocytes and immature hematopoietic cells, respectively. In lymph nodes and tonsils, Skp2 labeled active proliferating cells within germinal centers and scattered immunoblasts in interfollicular areas (D and E).