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Identification of isotype of rat anti-GBM antibodies in serum after Tx. Collagenase-digested F344 GBM preparations were coated in an ELISA plate, incubated with sera after Tx (1/25 dilution, closed bars) or normal LEW sera (open bars) and stained for immunoglobulin (sub)classes (mean of three sera ±SEM).
