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. 2002 Sep;161(3):781–786. doi: 10.1016/s0002-9440(10)64237-x

Figure 3.

Figure 3.

SV7 tert and tsc2ang1 cells express functional PDGFRβ. Subconfluent cultures of Tsc2ang1 or SV7tert cells were treated with PDGF-BB or vehicle for 8 minutes and aliquots of total cell lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and transferred to filters. One filter was incubated with anti-phosphotyrosine antibody 4G10 (αPY) and the secondary antibody against mouse immunoglobulin (α mouse) (A). A parallel filter was incubated with anti-PDGFRβ antiserum (α PDGFRβ), followed by the secondary antibody against rabbit immunoglobulin (α rabbit), at indicated dilutions. Immunoreactivity was detected by enhanced chemiluminescence (B).