Figure 10.

Effect of PDGF on stability of glucose-induced TGF-β1 mRNA. TGF-β1 mRNA was induced by addition of 25 mmol/L of d-glucose to confluent monolayer of HK2 cells for 48 hours. Subsequently transcription was inhibited by the addition of actinomycin D (5 μg/ml). Subsequently cells were exposed to 25 ng/ml of PDGF-AA (solid diamonds) or 5 mmol/L of d-glucose alone (open boxes) and total cellular RNA was isolated throughout the following 12 hours. Stability of TGF-β1 mRNA was determined by quantitative mimic PCR (B). Data represents mean ± SEM, n = 3; *, P < 0.05.