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Northern and Western blots for Ang-1 in hormone-treated HESCs. HESCs were treated for 10 days with control (C), E2 (E), MPA (P), or E2 + MPA (EP) as described in Materials and Methods. A: Northern blot of HESC-expressed Ang-1 mRNA, which was detected as described in Materials and Methods. Loading efficiencies were assessed by ethidium bromide staining of the 28S and 18S ribosomal RNA. B: Western blot of HESC-expressed Ang-1 protein was conducted as described in Materials and Methods.
