Figure 5.

Northern and Western blots for Ang-1 in hormone-treated HESCs under NX and HX. HESCs were treated for 10 days with control (C), E₂ (E), MPA (P), or E₂ + MPA (EP). At the end of this period, they were treated for a further 48 hours under NX or HX in the presence of the appropriate hormones as described in Materials and Methods. A: Northern blot of HESC-expressed Ang-1. Loading efficiencies were assessed by ethidium bromide staining of the 28S and 18S ribosomal RNA. B: Western blot of HESC-expressed Ang-1 protein.