Figure 8.

Western blots of SAPK/JNK and p38 after ROS treatment of HESCs. The activation (ie, phosphorylation) of p38 and SAPK/JNK in HESCs was detected after 30 minutes of ROS treatment as follows: lane 1, control; lane 2, H₂O₂ (50 μmol/L); lane 3, FeCl₂ (50 μmol/L) + H₂O₂; lane 4, FeCl₂ (50 μmol/L) + EDTA (50 μmol/L) + H₂O₂ (50 μmol/L); lane 5, FeCl₂ + H₂O₂ + DMSO (28 mmol/L). Parallel blots were probed for total MAPK (unphosphorylated) demonstrating equal levels of protein in all samples and no effect by DMSO.