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. 2002 Sep;161(3):929–938. doi: 10.1016/S0002-9440(10)64253-8

Figure 1.

Figure 1.

A: Protein level of EGF-R and activated EGF-R. Serum-starved HMVEC-D cultures were incubated for 15 minutes in medium (control) or medium with 10 ng/ml of bFGF, 10 ng/ml of VEGF, or 40 ng/ml of EGF. The levels of EGF-R protein (170 kd) and phosphorylated EGF-R (170 kd) were determined by Western blotting. Expression of EGF-R did not vary among the groups. In contrast, only endothelial cells incubated for 15 minutes with EGF expressed high levels of activated EGF-R. Densitometric quantification of the ratio between the Mr 170,000 phosphotyrosine-specific and the Mr 170,000 EGF-R-specific bands was compared in each case with the untreated cells whose ratio was defined as 1.0. B: Growth inhibition of human dermal endothelial cells treated in vitro with PKI 166. Cultures of HMVEC-D were incubated in control medium or in medium containing increasing concentrations (0 to 4 μmol/L) of PKI 166 in the presence or absence of bFGF (10 ng/ml) or EGF (10 ng/ml). The cells were incubated for 72 hours at 37°C when the number of metabolically active cells was determined using the tetrazolium 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenoltetrazolium assay. Bars, SD; *, P < 0.001.