Figure 5.

Hyperosmolarity induces up-regulation of IL-8 mRNA levels in HT-29 cells. Amiloride pretreatment (300 μmol/L) inhibits hyperosmolarity-induced IL-8 mRNA accumulation. Lanes 1 and 2: Control (isosmolar medium); lanes 3 and 4: hyperosmolarity (100 mmol/L of mannitol); lanes 5 and 6: amiloride and hyperosmolarity. GAPDH levels were not affected by both hyperosmolar and amiloride treatment. IL-8 and GAPDH mRNA levels were quantitated using reverse transcriptase-PCR. This figure is representative of three separate experiments.