Figure 7.

Amiloride pretreatment (300 μmol/L) inhibits hyperosmolarity-induced NF-κB DNA binding in HT-29 cells. NF-κB-specific complexes are indicated by arrows as determined by antibody supershifting. Hyperosmolarity was produced by the addition of mannitol (for a final concentration of 100 mmol/L) to isosmolar medium for 45 minutes. Control cells were treated with isosmolar medium. This figure is representative of three separate experiments.