Figure 8.

Hyperosmolar stimulation increases NF-κB-dependent transcriptional activity as compared to isosmolar stimulation. HT-29 cells were transiently transfected with a NF-κB-luciferase promoter construct, after which the cells were incubated for 16 hours with either hyperosmolar (100 mmol/L of mannitol) or isosmolar (control) medium. NF-κB-dependent transcriptional activity was determined using the luciferase assay (pNF-κB-Luc). This figure also shows that hyperosmolarity does not influence luciferase activity in cells transfected with an enhancerless construct (pTAL-Luc). Data are mean ± SEM of n = 14 to 16 wells from two separate experiments. **, P < 0.01.