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. 2002 Sep;161(3):969–978. doi: 10.1016/S0002-9440(10)64257-5

Figure 2.

Figure 2.

In situ hybridization detection of SIV vRNA+ cells and simultaneous detection of SIV and CD68 in rhesus macaque tissues. ISH was performed on lung tissues (M5499, A; M1799, B) with a pool of SIV-specific anti-sense riboprobes and representative fields are presented. The arrows in (A) denote SIV vRNA+ cells. Simultaneous ISH for SIV and IHC for CD68 was performed on lung tissue (M5299, C; M1799, E) and spleen (M5299, D; M5799, F). Productively infected cells are identified by the overlying collection of silver grains and CD68+ cells are identified by the brown precipitate. The arrows in C and D denote SIV vRNA+/CD68− cells. Parallel hybridizations with control sense riboprobes provided no autoradiographic signal. The bar in A represents 100 μm (A and B), whereas the bar in C represents 20 μm (C–F). Original magnifications, ×200 (A and B) and ×600 (C–F).