Figure 1.
NO donors dose dependently decrease keratinocyte release of IP-10, MCP-1, and RANTES, but not IL-8. Keratinocyte cultures were established from healthy patients (A, C, E, G) and patients with psoriasis (B, D, F, H). Keratinocytes were stimulated for 24 hours with 100 U/ml of IFN-γ and 50 ng/ml of TNF-α in the presence of different doses of GS-H (○), GS-NO (□), or NOR-1 (▪). Chemokine release was evaluated in the supernatants by ELISA. Data are expressed as mean ng/106 cells ± SD of triplicate cultures. Similar results were observed in keratinocyte cultures prepared from three healthy patients and three psoriatic patients.