Figure 7.

NO donors reduce luciferase expression driven by NF-κB and STAT-1 transcription factors in transiently transfected keratinocytes activated with IFN-γ and TNF-α. Keratinocytes obtained from healthy patients (A, C, E) and from psoriatic patients (B, D, F) were transfected with 1 μg of pNF-κB-Luc (A, B), pGAS-Luc (C, D), or pAP-1-Luc (E, F) plasmids, and then treated as indicated. After 24 hours keratinocytes were lysed to determine β-galactosidase and luciferase activities. Results are expressed as relative luciferase activity (RLA), which represents the luciferase activity normalized to the β-galactosidase activity. Values are the mean ± SD of four replicates for each experimental condition.