Table 1.
NO Donors Do Not Induce Keratinocyte Apoptosis or Necrosis in Vitro
| Treatment* | % Annexin V+ cells | % PI+ cells | % Annexin V+/PI+ cells |
|---|---|---|---|
| None | 0.6 ± 0.4 | 6.1 ± 2.5 | 0.9 ± 0.3 |
| IFN-γ/TNF-α | 2.6 ± 0.8† | 6.6 ± 1.4 | 5.1 ± 0.4† |
| GS-NO | 0.8 ± 0.3 | 7.6 ± 0.9 | 1.6 ± 0.5 |
| NOR-1 | 1.1 ± 0.4 | 7.3 ± 0.6 | 2.6 ± 0.6 |
| IFN-γ/TNF-α + GS-NO | 3.6 ± 1.4† | 8.6 ± 1.2 | 6.8 ± 1.1† |
| IFN-γ/TNF-α + NOR-1 | 3.8 ± 0.7† | 8.2 ± 0.4 | 6.6 ± 0.5† |
*Keratinocyte cultures were left untreated or stimulated with 100 U/ml of IFN-γ and 50 ng/ml of TNF-α and/or 2.5 mmol/L of GS-NO or NOR-1. After 24 hours, cells were stained with propidium iodide and anti-annexin V antibody, and then analyzed by flow cytometry. Results are expressed as mean percentage ± SD of positive cells from three independent experiments.
†P < 0.05 compared to untreated keratinocytes.